Variation of starch granules in diploid species of the genus Avena L.

  • Romuald Kosina Plant Speciation Group, Institute of Environmental Biology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
  • Anna Grabińska Plant Speciation Group, Institute of Environmental Biology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

Abstract

Composite starch granules, the main product of assimilation in oat endosperm, were analyzed in the accessions of both wild and cultivated diploid species of the genus Avena. Simple starch granules are mostly synthesized in the outer parts of the endosperm tissue. The size of sub-grains in a composite granule does not depend on a wild or cultivated status of the species. Inter-specific variation in the size differences of composite granules is large. Also, a broad variation has been detected for granules analyzed in a Lugol’s solution or polarizing light. This analysis revealed a difference between the synthesis of amylopectin versus amylose in a granule. Examples of occurrences of low levels of amylopectin synthesis are provided.

References

Baum B.R. 1977. Oats: wild and cultivated. A monograph of the genus Avena L. (Poaceae). Thorn Press Limited, Ottawa.
Baum B.R., Bailey L.G. 1987. A survey of endosperm starch granules in the genus Hordeum: A study using image analytic and numerical taxonomic techniques. Canad. J. Bot. 65: 1563–1569.
Broda B. 1971. Metody histochemii roślinnej. PZWL, Warszawa.
Burton R.A., Jenner H., Carrangis L., Fahy B., Fincher G.B., Hylton C., Laurie D.A., Parker M., Waite D., van Wegen S., Verhoeven T., Denyer K. 2002. Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity. Plant J. 31: 97–112.
Edwards A., Vincken J-P., Suurs L.C.J.M., Visser R.G.F., Zeeman S. 2002. Discrete forms of amylose are synthesized by isoforms of GBSSI in pea. Plant Cell 14: 1767–1785.
Grabińska A. 2008. Zmienność mikrostrukturalna owocu diploidalnych gatunków Avena L. MSc thesis. University of Wroclaw, Wroclaw.
Klemsdal S.S., Kvaale A., Olsen O.A. 1986. Effects of the barley mutants Risø 1508 and 527 high lysine genes on the cellular development of the endosperm. Physiol. Plant. 67: 453–459.
Kosina R. 2007. Some topics of the grass mosaics. In: Frey L. (ed.), Biological issues in grasses: 159–167. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.
Kosina R. 2009. On polymorphism of starch grains in the grass endosperm. In: Frey L. (ed.), Grass Research: 109–118. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.
Kosina R., Tomaszewska P. 2011a. Contribution on Avena (Poaceae) amphiploids endosperm. In: Frey L. (ed.), Advances in grass biosystematics: 119–127. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków.
Kosina R, Tomaszewska P. 2011b. On wheat and Brachypodium distachyon caryopsis. Ann. Wheat Newslett. 57: 250.
Kosina R., Tomaszewska P., Zając D. 2015. Polymorphism of starch granules in endosperm of some species and amphiploids of the Triticeae L. tribe. Ann. Wheat Newslett. 61: 47–49.
Patron N.J., Smith A.M., Fahy B.F., Hylton C.M., Naldrett M.J., Rossnagel B.G., Denyer K. 2002. The altered pattern of amylose accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase I with a deletion in the 5’-non-coding region. Plant Physiol. 130: 190–198.
Tester R.F., Karkalas J., Qi X. 2004. Starch – composition, fine structure and architecture. J. Cereal Sci. 39: 151–165.
Verhoeven T., Fahy B., Leggett M., Moates G., Denyer K. 2004. Isolation and characterisation of novel starch mutants of oats. J. Cereal Sci. 40: 69–79.
Wilson J.D., Bechtel D.B., Todd T.C., Seib P.A. 2006. Measurement of wheat starch granule size distribution using image analysis and laser diffraction technology. Cereal Chem. 83: 259–268.
Composite starch granules: A – Avena pilosa; B – A. eriantha; C, D – A. wiestii. A-D are taken under a light microscope; A-C under a polarised light; D – shows the granules stained in a Lugol’s solution. Microscopic magnification: A-C – ×320; D – ×800.
Published
2016-10-01
How to Cite
KOSINA, Romuald; GRABIŃSKA, Anna. Variation of starch granules in diploid species of the genus Avena L.. Modern Phytomorphology, [S.l.], v. 10, n. Suppl., p. 9-12, oct. 2016. ISSN 2227-9555. Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/2>. Date accessed: 27 mar. 2017. doi: https://doi.org/10.5281/zenodo.159036.
Section
Correspondences

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